Cloudspec: combined extinction/absorption spectrophotometer
The CloudSpec UV-Vis spectrophotometer, developed by MaramaLabs, solves the problem of light-scattering from cloudy samples by placing the sample inside an integrating sphere. The unique optical design has two pathways in which light interrogates the sample. The first is the standard “UV-Vis” configuration that yields the extinction spectrum of the sample, while the second pathway uses the integrating sphere to measure the absorption spectrum. This method is called Combined Extinction Absorbance Spectroscopy, and has a number of advantages:
- No Filtering of Samples; The integrating sphere eliminates the effects of scattering/turbidity.
- Interchangeable Cuvettes; Choose from 1mm, 2mm or 10mm depending on application.
- Quantitative Results; Calibration algorithm reports absorbances for a 10mm cuvette
- Detection down to 0.001A; Path length enhancement allows ultra-low limits of detection.
- Simple to use software; Familiar to any user of UV-Vis spectrophotometer
- 3 Spectra in one measurement; Extinction, absorption and scattering (calculated) output automatically
Due to the complex way that light interacts with a sample inside an integrating sphere, the spectra obtained cannot be interpreted using the standard Beer-Lambert law approach.
Many approaches have been developed to “calibrate” an integrating sphere device, however most require running lengthy sets of standard measurements.
MaramaLabs have a developed a powerful algorithm that corrects the spectra obtained so they
are normalised to a standard path length. No lengthy empirical calibration needs to be run to
obtain quantitative UV-Vis spectra. The CloudSpec calibration algorithm ensures that all spectra obtained can be directly interpreted as what would be obtained in a standard 10mm path length cuvette. Therefore the Beer-Lambert law is revived and concentration curves can be developed.
|Instrument Type||Single-beam, array detector|
|Wavelength Range||250-800 nm|
|Resolution||~ 2 nm|
|Absorbance Range (10 mm Cuvette)||0.005 A – 2 A (extinction spectrum) 0.001 A – 1.5 A (Absorbance Mode)|
|Cuvettes||Quartz 10 mm (standard) 1 mm (optional)|
|Detector||2048 Pixel CCD|
|Light Source||Pulsed Xenon|
|Sample Volume Required||1 mL (10 mm cuvette)|
|Measurement Time (Typ)||5 – 15 seconds|
|Dimensions||45 x 30 x 15 cm (w x d x h)|