CloudSpec UV-Vis Spectrophotometer – for turbid samples

Absorbance measurements of highly scattering/turbid samples

  • 250-800 nm
  • 0.001 A – 1.5 A
  • ~2 nm spectral bandwidth

UV-Vis spectroscopy is a simple, yet powerful, technique that uses light in the ultra-violet and visible range to obtain physical and chemical information of liquid samples, including molecular concentration. There are many instruments on the market that perform these measurements for simple solutions. The problem for many real world applications however, is the solutions often contain particles/colloids/emulsions that induce scattering, resulting in cloudy samples that require filtering/centrifugation/extraction before UV-Vis measurements can be performed, but what if those particles are a critical part of the sample being characterised. The CloudSpec’s unique optical design allows to measure both extinction and absorbance allowing UV-Vis measurements to be directly performed on previously impossible samples.

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Cloudspec: combined extinction/absorption spectrophotometer

The CloudSpec UV-Vis spectrophotometer, developed by MaramaLabs, solves the problem of light-scattering from cloudy samples by placing the sample inside an integrating sphere. The unique optical design has two pathways in which light interrogates the sample. The first is the standard “UV-Vis” configuration that yields the extinction spectrum of the sample, while the second pathway uses the integrating sphere to measure the absorption spectrum. This method is called Combined Extinction Absorbance Spectroscopy, and has a number of advantages:

  • No Filtering of Samples; The integrating sphere eliminates the effects of scattering/turbidity.
  • Interchangeable Cuvettes;  Choose from 1mm, 2mm or 10mm depending on application.
  • Quantitative Results; Calibration algorithm reports absorbances for a 10mm cuvette
  • Detection down to 0.001A; Path length enhancement allows ultra-low limits of detection.
  • Simple to use software; Familiar to any user of UV-Vis spectrophotometer
  • 3 Spectra in one measurement; Extinction, absorption and scattering (calculated) output automatically

Calibrated Absorbance

Due to the complex way that light interacts with a sample inside an integrating sphere, the spectra obtained cannot be interpreted using the standard Beer-Lambert law approach.
Many approaches have been developed to “calibrate” an integrating sphere device, however most require running lengthy sets of standard measurements.

MaramaLabs have a developed a powerful algorithm that corrects the spectra obtained so they
are normalised to a standard path length. No lengthy empirical calibration needs to be run to
obtain quantitative UV-Vis spectra. The CloudSpec calibration algorithm ensures that all spectra obtained can be directly interpreted as what would be obtained in a standard 10mm path length cuvette. Therefore the Beer-Lambert law is revived and concentration curves can be developed.


Instrument TypeSingle-beam, array detector
Wavelength Range250-800 nm
Resolution~ 2 nm
Absorbance Range (10 mm Cuvette)0.005 A – 2 A (extinction spectrum) 0.001 A – 1.5 A (Absorbance Mode)
CuvettesQuartz 10 mm (standard)                       1 mm (optional)
Detector2048 Pixel CCD
Light SourcePulsed Xenon
Sample Volume Required1 mL (10 mm cuvette)
Measurement Time (Typ)5 – 15 seconds
Dimensions45 x 30 x 15 cm (w x d x h)

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