The DeltaPro is a remarkably affordable, easy to use filter-based lifetime fluorometer that still offers capabilities found in far more expensive and complex systems. DeltaPro’s simplicity makes it the ideal system for a multi-user facility with researchers who need to measure lifetimes, or a combined research/ teaching lab. But don’t let the price and approachability fool you. The DeltaPro is a serious research instrument capable of picosecond lifetime measurements. With the addition of SpectraLED excitation sources it instantly becomes a phosphorimeter. Our proprietary optimized performance optics, sources and detectors mean that measurement of weakly emitting samples becomes routine. Finally, the DeltaPro is supported by our analysis software that gives you access to the same full array of lifetime analysis modules as our most sophisticated systems.
Why measure fluorescence lifetimes?
Fluorescence is an ideal nanoscale probe, as it takes place on the nanosecond timescale and can be influenced by molecular processes occurring on the nanometre range. The emission lifetime of a
fluorophore can be highly influenced by its environment or the presence of other interacting molecules.
Thus fluorescence lifetime is useful in measuring:
• Changes in the nanoenvironment (viscosity, pH, polarity, solvation)
• Size and shape of molecules
• Molecular interactions
• Inter- and intramolecular distances
• Kinetic and dynamic rates
• Resolution of molecular mixtures
The extra specificity of the fluorescence lifetime allows easy discrimination against scattered excitation and background fluorescence. For example, determination of Förster resonance energy transfer (FRET) is simpler using the fluorescence lifetime, as are quenching and fluorescence anisotropy measurements, allowing more parameters to be recovered. Unlike steady-state, lifetime is also an absolute measurement and is independent of sample concentration, label loading/binding uniformity, and fluctuations in excitation intensity.
The new Delta series of fluorescence TCSPC lifetime fluorometer are the fastest, most flexible, and most affordable lifetime fluorometer solutions available. The Delta series includes the DeltaPro, the very affordable and simple to use filter-based lifetime system with performance that rivals most high end systems. For the ultimate flexibility and upgradability, DeltaFlex, our modular system, seamlessly integrates monochromators, polarizers, the widest array of sources (LEDs, laser diodes, supercontinuum lasers) and detectors (including NIR). This enables lifetimes from 25 ps to 1 sec, over wavelengths spanning the UV to NIR, to be measured.
The culmination of over 40 years of lifetime experience, Delta series highlights include: the fastest sources (up to 100 MHz), the widest lifetime ranges (ps to sec), virtually unlimited configurability with our new F-Link plug-and-play architecture and advanced lifetime analysis software. The Delta series is truly the next generation of fluorescence lifetime systems.