Duetta Fluorescence and Absorbance Spectrometer

A Superior Spectrometer Concept

The Future of Fluorescence

  • Two-in-one Fluorescence and Absorbance Spectrometer
  • UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm
  • Full 3-D Fluorescence EEM Acquisition in Less Than One Second
  • Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman
  • Automatic Correction for Primary and Secondary Inner Filter Effects (IFE)
  • High Fidelity Molecular Fingerprinting with Unique A-TEEM™ (Absorbance-Transmittance Excitation Emission Matrix) Technology
  • Millisecond CCD Detection of Entire Fluorescence Spectrum

Fluorescence and Absorbance in one Spectrometer!

Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.

Fluorescence in the Blink of an Eye!

Duetta’s ultra-fast, state-of-the-art CCD technology allows it to outperform, by orders of magnitude, any competitor using PMTs. It’s the fastest bench-top fluorometer in the world, and the only fluorometer that can acquire a full spectrum in less than 100 milliseconds! This state-of-the-art CCD technology also extends NIR wavelength detection to 1,100 nm, well beyond the limit of a standard PMT fluorometer.

Highest Fluorescence Sensitivity in its Class

Having the highest level of fluorescence sensitivity means that Duetta’s optimized design lets you measure lower concentrations of a sample, and provides better quality data.

EzSpec™ Touch-Screen Software!


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Fluorescence and Absorbance Spectrometer

Duetta™ is a 3-in-1 spectrofluorometer that combines the functions of fluorescence and absorbance spectrometers simultaneously. Thanks to its high-speed, built-in CCD detector, the Duetta can acquire a full spectrum from 250 nm to 1,100 nm in less than one second, making it the fastest fluorescence spectrometer on the market.

Duetta uses A-TEEM™ technology to acquire absorbance, transmission, and fluorescence EEMs simultaneously – a true 3-in-1 instrument that corrects for the inner filter effect on the fly.

Data acquisition is more accurate because measurements are taken from the same spot without moving the sample and repeating the exercise.

To aid in the use of the Duetta and teach fluorescence spectroscopy principles in an undergraduate teaching laboratory setting, we have made a series of education labs. Designed to teach some of the most important concepts in fluorescence spectroscopy, these education labs can be used as is, or tailored to fit the needs of any undergraduate laboratory. To learn more about these labs, please visit Duetta Education Labs.


Acquisition ModesAbsorbance and Fluorescence Spectrometer
Fluorescence SensitivityWater Raman SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits
Spectral Acquisition Rate510,000 nm/min
EEM Acquisition RateAs fast as 1 second (sample and wavelength dependent)
A-TEEM Acquisition RateAs fast as 30 seconds (sample and wavelength dependent)
Fluorescence DetectorCCD/Spectrograph
Fluorescence Detector Range250 to 1,100 nm
Fluorescence Bandwidth1, 2, 3, 5,10, 20 nm (excitation and emission)
Light Source75 W Xenon arc lamp. Dedicated cartidge for snap-in replacement
Excitation/Absorbance Wavelength Range250 to 1,000 nm
Absorbance DetectorSilicon Photodiode
Absorbance Detector Range250 to 1,000 nm
Absorbance Bandwidth1, 2, 3, 5,10, 20 nm
Absorbance Range0 to 2 A
Absorbance Accuracy+/- 0.02 A
Wavelength Accuracy+/- 1 nm
Computer RequirementsWindows 10 or 11 64-bit OS, laptop or PC with at least 1 USB port (more for additional accessories)
Dimensions (W x D x H)17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm
Weight45 pounds; 20.4 kg
Polarizers (Time of Purchase)Not AvailableYes (280 to 750 nm)

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