Open Research Platform for Fast and Easy Kinetic Interaction Monitoring
OpenPlex SPR Imaging platform is the ideal solution for the development of label-free multiplexed bioassays and biomolecules detection. It uses Surface Plasmon Resonance (SPR) imaging to track the real-time binding of molecules on multiple spotted areas located on a sensor chip. Typical applications include biosensor development, pathogen detection, water monitoring, nanotechnology and surface characterisation.
SPRi Technology for Real-Time Label-Free Biomolecular Interactions
OpenPlex uses Surface Plasmon Resonance imaging (SPRi) to follow label-free binding events in real-time.
SPR measures modifications of the refractive index located at the surface of a sensor chip, which can be correlated to mass variations. Binding events create local changes of the refractive index, and therefore changes of reflectivity (∆R) of the incident light at a fixed angle. Thanks to the imaging capability, multiple interactions can be monitored simultaneously.
Multiplexing: Performance and Speed Combined
The imaging configuration of OpenPlex dramatically enhances the throughput of conventional SPR.
Ligands molecules are immobilized in an array format onto the sensor chip surface and are screened against the interacting partner in the sample solution.
Running hundreds of molecules in one biochip without labelling will shorten your time-to-result significantly!
Instant Information about Molecular Interactions From Real-Time Display of Spots
Binding events are followed in real-time on the kinetic curve (sensorgram) and also on the SPRi difference image. White areas on the SPRi difference image correspond to areas where binding has occurred – giving a clear Yes/No answer to molecule detection.
Biosensor Development – Biomolecule Detection
Gold nanostructures or quantum dots can increase OpenPlex sensitivity for detecting biomolecules. Here, the combination of nanotechnologies and SPRi allows the detection of bacterial RNA (picomolar concentrations).
OpenPlex can measure affinity and kinetic rates involving various types of biomolecules including DNA, RNA, proteins, peptides, antibodies, oligossacharides, and more.
Biofilm Dynamics Monitoring
The unique imaging capability of SPRi brings you the image function so you can see in real-time any modification occurring on the sensor chip surface. For example, biofilms dynamics can be analysed from different surface coatings simultaneously.
Simplicity and Flexibility Combined
OpenPlex can work with different optional flow cells to give you more flexibility. Two possibilities are offered:
one flow cell with temperature control and other with different configurations, which can be used to work in
a static mode (no flow) or to combine SPRi with other techniques such as Fluorescence or Electrochemistry.