How it works
The work flow for Particle Finder is extremely simple and intuitive. The module is an integrated part of the powerful LabSpec 6 software, and is fully linked to related modules for data acquisition, processing, analysis and display.
1. Particle location
Simple processing of the sample optical image quickly locates the particles, creating a binary image which clear highlights the particle positions. Full morphological filtering tools are included to ensure truly meaningful location. The optical image can cover any user specified area, using the integrated video montage/mosaic tool, allowing millimetre or centimetre areas to be covered.
2. Statistical analysis
Once the particles are located, particle shape and size parameters are calculated for each particle, including a statistical summary. Histograms of the parameters (including area, perimeter, axes, ellipse ratio, circularity and brightness) are available for easy visualization.
3. Particle selection
Initially, all located particles are set for Raman analysis. However, they can be filtered according to the statistics results, allowing only particles which correspond to a specific size/shape to be analysed. For example, only particles within a certain size range can be marked for Raman characterisation.
4. Raman acquisition
The full capabilities of the Raman instrument can be utilised for the Raman acquisition stage, including choice of laser wavelength, spectral resolution, confocality and mapping. Such configurable experiments ensure that optimised results can be obtained from varying sample sets with ease. The Raman spectra are displayed in a table together with the statistical parameters.
5. Data analysis
Once the Raman data has been acquired, it is automatically linked to the individual particles in the binary image. The spectra are now available to be processed, analysed and displayed according to the full powerful functionality of LabSpec 6.
Spectra can be exported to the HORIBA Edition KnowItAll® database searching tool for advanced spectrum matching to known materials.