Simultaneous Multi-Laser Nanoparticle Tracking Analysis (NTA)
Analyzing nanoparticles such as colloids, viruses, proteins, and extracellular vesicles (EV) is inherently challenging. These particles are too small to image with visible light and must be imaged by laborious electron or scanning microscopy. Dynamic light scattering and laser diffraction have been successfully used to determine particle size and size distribution. These techniques are fast and accurate for certain samples, however, as they are ensemble techniques, high resolution distribution information cannot be obtained.
The ultramicroscope and conventional single-laser nanoparticle tracking have been used with only partial
success since the wide range of sizes present in many samples means that scattering from the largest particle
is bright enough to saturate the detector and eliminate any hope of learning about smaller particles. This known
problem is discussed in ISO 19430:2016 Particle size analysis — Particle tracking analysis (PTA) method where
it states, “…Sample polydispersity affects the ability to track and therefore analyse different size fraction in
particle number-size distribution.” To overcome these long-standing problems, the ViewSizer 3000 integrates
three simultaneous operating lasers with independently adjustable power to enhance particle detection from 10
nanometres to 15 microns.
The ViewSizer 3000 exploits advanced hardware and software to visualize scattered light from individual
particles in suspension. This data is then used to determine particle movement and infer particle size using
the Stokes-Einstein relationship. Furthermore, since the illuminated sample volume is well known along with the number of particles imaged, particle number concentration is readily determined. Thus, from a single measurement, two critical pieces of information are determined: particle size distribution and particle concentration even for polydisperse samples.
|Measurement Range||10 nm to 15 μm|
|Typical Sample Volume||350 µL to 2.5 mL|
|Typical Sample Concentration|
|10^5 – 10^9 particles/mL|
|Sample Temperature Range|
|10° C to 50° C +/- 0.1° C|
|Dimensions||55 cm W x 66 cm D x 35 cm H|
|Operational Environment||15° C to 30° C with < 85% RH|